TABLE 2.
Effects of mutations in lon, rcsB, and hns on expression of the rcsA90::lacZ transcriptional fusion and expression of capsule
| Straina | Relevant genotype | β-Galactosidase activityb | Mucoidyc |
|---|---|---|---|
| DDS90 | lon+ rcsB+ hns+ | 23 | − |
| JT2058 | lon+ rcsB hns+ | 27 | − |
| WE28 | lon+ rcsB+ hns | 264 | + |
| WE30 | lon+ rcsB hns | 242 | − |
| JT2046 | Δlon rcsB+ hns+ | 2,415 | + |
| JT2056 | Δlon rcsB hns+ | 21 | − |
| WE29 | Δlon rcsB+ hns | 2,664 | + |
| WE31 | Δlon rcsB hns | 197 | − |
a
All strains are MC4100 derivatives carrying an rcsA90::lacZ fusion integrated at the λatt site.
b
β-Galactosidase assays were carried out as described by Miller (17). Values (in Miller units) are averages of three independent assays.
c
Mucoidy was assessed visually. −, nonmucoid; +, mucoid.