TABLE 3.

Effects of the putative Rcs box on expression of rcsA::lacZ and cps::lacZ fusions

Relevant genotype	β-Galactosidase activitya
	rcsA90::lacZb	rcsA109::lacZc	cps103::lacZb	cps41::lacZc
lon+ rcsA+ rcsB+	4	220	7	17
Δlon rcsA+ rcsB+	1,189	176	139	20
Δlon rcsA rcsB+	5	219	6	8
Δlon rcsA+ rcsB	5	203	3	8
Δlon rcsA rcsB	5	234	12	24

^aβ-Galactosidase assays were carried out as described by Miller (17). Values (in Miller units) are averages of three independent assays. All strains are MC4100 derivatives carrying the described fusion integrated at the λatt site. 

^bThese fusions contain the putative Rcs box: DDS90 (lon⁺ rcsA⁺ rcsB⁺ rcsA₉₀::lacZ), WE1003 (lon⁺ rcsA⁺ rcsB⁺ cps₁₀₃::lacZ), JT2046 (Δlon-510 rcsA⁺ rcsB⁺ rcsA₉₀::lacZ), WE1023 (Δlon-510 rcsA⁺ rcsB⁺ cps₁₀₃::lacZ), JT2055 (Δlon-510 rcsA72::ΔTn10 rcsB⁺ rcsA₉₀::lacZ), WE1053 (Δlon-510 rcsA72::ΔTn10 rcsB⁺ cps₁₀₃::lacZ), JT2058 (lon⁺ rcsA⁺ rcsB62::ΔKan rcsA₉₀::lacZ), WE1063 (Δlon-510 rcsA⁺ rcsB62::ΔKan cps₁₀₃::lacZ), JT2059 (Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan rcsA₉₀::lacZ), and WE1083 (Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan cps₁₀₃::lacZ). 

^cThese fusions do not contain the putative Rcs box: WE1002 (lon⁺ rcsA⁺ rcsB⁺ rcsA₁₀₉::lacZ), WE1004 (lon⁺ rcsA⁺ rcsB⁺ cps₄₁::lacZ), WE1022 (Δlon-510 rcsA⁺ rcsB⁺ rcsA₁₀₉::lacZ), WE1024 (Δlon-510 rcsA⁺ rcsB⁺ cps₄₁::lacZ); WE1052 (Δlon-510 rcsA72::ΔTn10 rcsB⁺ rcsA₁₀₉::lacZ), WE1054 (Δlon-510 rcsA72::ΔTn10 rcsB⁺ cps₄₁::lacZ), WE1062 (Δlon-510 rcsA⁺ rcsB62::ΔKan rcsA₁₀₉::lacZ), WE1064 (Δlon-510 rcsA⁺ rcsB62::ΔKan cps₄₁::lacZ), WE1082 (Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan rcsA₁₀₉::lacZ), and WE1084 (Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan cps₄₁::lacZ).