Figure 1.

Colonic mast cells and gut bacteria in mice with human microbiota.

a: Representative micrographs of immunofluorescent staining for mast cells-tryptase (red), neural fibers-Tuj-1 (green) in HC and IBS mice. b: Number of tryptase positive mast cells and number of mast cells in close proximity to nerves in HC (n = 9) and IBS (n = 42–44) mice. c: FISH staining of 16S rRNA (EUB-16S rRNA) in the colon of HC or IBS mice. Dapi in blue and EUB-16S-rRNA in red.d: Representative pictures of RNAscope staining of mast cells (tryptase, Tpsab1, red) and bacteria (16S rRNA, EUB-16SrRNA, blue). e: Quantification of RNAscope staining of mast cells and bacteria in HC (n = 5) and IBS (n = 11) mice. The data were analyzed with Mann–Whitney test (b, e).