TABLE 3.
Cells with PHA as a percenta of total cells following growth on different carbon sources
| Substrate(s) (no. of C atoms) | % Cells with PHA
|
||||
|---|---|---|---|---|---|
| B. megaterium | P. oleovoransb | P. putida GPp104(pSUP104)c | P. putida GPp104(pGM107)d | P. putida GPp104(pDR1)e | |
| LB | 100 | 0 | 0 | 90 | 0 |
| LB–1% glucose | 100 | 0 | 0 | 92 | 0 |
| M9–12 mM caproate (6) | No growth | 88 | 0 | 100f | 0 |
| M9–12 mM octanoate (8) | No growth | 90 | 0 | 92 | 0 |
100%, PHA in all cells; 0%, no PHA in any cell; data averaged from more than five fields of each of three different cultures; error <5%.
Positive control.
Negative control, vector only.
phaPnQRBC (n indicates presence of N terminus only).
phaC.
Cell shape distorted by large quantity of PHA.