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. 2022 Jul 18;9:865330. doi: 10.3389/fmed.2022.865330

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Copyright © 2022 Gomes de Castro, Lopes da Silva, Nahar dos Santos, Leal, de Pinho Pereira, Alvim and Esquenazi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of regulatory T cells producing TGF-β. Evaluation of CD4+ and CD8+ Treg frequency in blood samples in vitro with no stimuli. Representative histogram from strategy analysis, cells were gated in regulatory T cell phenotype (A); Frequency of CD4+CD25+Foxp3+TGF-β+ (B); CD8+CD25+Foxp3+TGF-β+ (C); Ratio of CD4+CD25+Foxp3+TGF-β+/CD8+CD25+Foxp3+TGF-β+ (D). Each symbol represents an individual studied according to the group; the horizontal lines represent the median. One-way Anova was used for statistical analysis where, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001. T1R, Type 1 Reaction; BL, borderline lepromatous; T2R, Type 2 Reaction; LL, polar lepromatous patients; HV, healthy volunteers.