Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 18;13:923031. doi: 10.3389/fimmu.2022.923031

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Jia, Ga, Liu, Yang, Wang, Guo, Ma, Liu, Li, Tang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

SPINK1-regulated chemoresistance, anti-apoptosis and SPINK1-mAb targeting experiments. (A, B) SPINK1 enhanced chemoresistance in stable SPINK1-OE HepG2 and SPINK1-OE H22 cells following 24 h of exposure to 5-FU; (C) Decreased chemoresistance to 5-FU in SPINK1 knockdown Hep3B cells 24 h post-5-FU treatment. (D) Examination of apoptosis-related molecules including main pro- (Caspase 3, Bid and Bad) and anti-apoptotic regulators (Bcl-XL and Bcl-2) using Western blot in SPINK1-OE HepG2 and empty-vector control cells with or without 5-FU addition. (E, F) Targeting SPINK1 using anti-human SPINK1- mAb in SPINK1- OE H22 and - OE HepG2 cells, with IgG as an isotype control. Error bars represent mean ± standard deviation of independent experiments or the experiments performed in triplicates either in 6-well or 96-well plates. OD, optical density; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.