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Primer extension analyses of H. pylori RNAs. The elongated primers are indicated by arrows and labeled “P.” Subscript numbers refer to the downstream ORF as designated by Tomb et al. (33). Total RNA was extracted from wild-type H. pylori G27 (lanes +) or from the flgR isogenic mutant (lanes −), annealed to specific primers, and elongated with reverse transcriptase as detailed in Materials and Methods.
