Figure 4.

Reconstruction of splicing regulation in late midbrain development. (A) Modules of cell state–associated exons identified in postnatal day 90 (P90) cells, at different stages of development. Heatmaps show the correlation matrix among the P90 module A and B exons at each time point. As a measure of structure, for each correlation matrix, we show its first eigenvalue divided by the sum of all eigenvalues. PCA plots show the splicing patterns of the exons of the module in the cells of each time point. (B) Correlation of splicing factor expression with the observed $\overline{\mathrm{\Psi }}$ of exons from P90 modules A and B at different stages of development. An asterisk indicates significant difference between the exons of module A and B (Wilcoxon rank test, FDR ≤ 0.05) and a mean difference larger than 0.1 between the two modules. Exon 4 of Noval (in module B) is highlighted in red in the correlation plot of Noval gene expression. (C) Gene expression of Noval and splicing of Noval exon 4 in mouse midbrain neurons.