FIG. 1.

(A) Genetic background of the parental strain hliNG. This strain contains the native hliA and nblA genes, an autonomously replicating plasmid derived from pC4B (39) that confers kanamycin (kan) resistance to the cyanobacterium, the nblA gene fused to the hliA promoter and 5′ untranslated region at the initiator AUG codon (on the plasmid), and an hliA-GUS chimeric gene (see Materials and Methods), contiguous to the gene for spectinomycin (spec) and integrated into the cyanobacterial genome. (B) Pigmentation of wild-type (WT) cells and the hliNG strain in liquid medium in LL and HL.