TABLE 2.
TetRC58 controls expression of tetAC58 and tetRC58
| Strain | tetRC58 source | β-Galactosidase activitya (tetAC58::lacZ)
|
β-Glucuronidase activitya (tetRC58::uidA)
|
||
|---|---|---|---|---|---|
| No ara | With ara | No ara | With ara | ||
| NT1TcR1(pZLOP1, pDLB4) | None | 77 | 85 | 53 | 45 |
| NT1TcR1(pZLOP1, pDLB4-tetR) | pDLB4-tetR | 65 | 2 | 18 | 6 |
| NT1(pZLOP1, pDLB4) | Chromosome | 19 | 14 | 14 | 20 |
| NT1(pZLOP1, pDLB4-tetR) | Chromosome/pDLB4-tetR | 25 | 2 | 14 | 7 |
| DH5α(pZLOP1, pDLB4) | None | 2,213 | 2,243 | NTb | NT |
| DH5α(pZLOP1, pDLB4-tetR) | pDLB4-tetR | 1,678 | 29 | NT | NT |
a
β-Galactosidase and β-glucuronidase activities are expressed as units per 109 CFU as described in Materials and Methods. Activities were determined in cultures with or without 0.4% arabinose (ara) added as an inducer.
b
NT, not tested (E. coli DH5α has an endogenous β-glucuronidase activity).