TABLE 1.

Strains and plasmids

Plasmid or bacterial strain	Description	Reference or source
Plasmid
pET23b	Phage T7 RNA polymerase-based protein expression vector	13, Novagen
pET28b	Phage T7 RNA polymerase-based protein expression vector	13, Novagen
pAU111	pET23b with vanR NdeI-HindIII cassette inserted at the NdeI and HindIII sites in the vector multiple cloning site in frame with the 6 His tag sequence present	This work
pAU112	pET23b with vanS NdeI-BamHI cassette inserted at NdeI and BamHI in the multiple cloning site (no His tag)	This work
Bacterial strain
E. faecium A634	VanA reference strain, source of vanR, vanS, and vanSc DNA sequence	R. F. Moellering
E. coli EAU109	E. coli BL21DE3-pLysS transformable recipient strain with phage T7 RNA polymerase under the control of LacI and LysS	Novagen
EAU110	E. coli BL21DE3-pLysS + pET23b VanS-negative control strain	This work
EAU111	E. coli BL21DE3-pLysS + pAU111 production strain for VanR	This work
EAU112	E. coli BL21DE3-pLysS + pAU112 production strain for VanS	This work