FIG. 8.

Relationship between the activity of glycerol kinase and the extent of inhibition of glycerol uptake by IIA^LacS (■) and IIA^LacS-6H(I548E/G556D) (▾). Cells were grown as described in the legend to Fig. 7. The data are from several independent experiments that reflect partial induction by glycerol for 15, 20, or 30 min. The glycerol kinase activity was measured as [¹⁴C]glycerol uptake rate in the absence of 2-deoxyglucose, and the inhibition of [¹⁴C]glycerol uptake was measured from the ratio of the rates in the presence and absence of 10 mM 2-deoxyglucose.