Figure 6.

Circ_0078767 inhibits OS progression by regulating KLF9.

(a). Western blot was used to detect the expression level of KLF9 in MG63 cells transfected with KLF9 overexpression plasmid and control plasmid.(b&c). Western blot was used to detect the expressions of KLF9 mRNA and protein in MG63 cells after transfection of si-circ_0078767#2 or/and KLF9 overexpression plasmid.(d). Detection of MG63 cell proliferation after transfection of si-circ_0078767#2 or/and KLF9 overexpression plasmid by MTT assay.(e). Detection of MG63 cell migration after transfection of si-circ_0078767#2 or/and KLF9 overexpression plasmid by scratch wound healing assay. Scale bars, 100 μm.(f). Detection of MG63 cell invasion after transfection of si-circ_0078767#2 or/and KLF9 overexpression plasmid by Transwell invasion assay. Scale bars, 250 μm.(g). Western blot was used to detect the expression levels of E-cadherin and N-cadherin in MG63 cells transfected with si-circ_0078767#2 or/and KLF9 overexpression plasmid.(h). TUNEL assay was used to detect the apoptosis of MG63 cells after transfection of si-circ_0078767#2 or/and KLF9 overexpression plasmid. Scale bars, 250 μm.Each experiment was carried out in triplicate. **P < 0.01, ***P < 0.001.