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. 2022 Jun 26;13(6):14159–14174. doi: 10.1080/21655979.2022.2083855

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — MiR-331-3p targeted MGAT1 (a) The binding site between miR-331-3p and the MGAT1 3’UTR was identified using Targetscan. (b) Confirmation of interaction between miR-331-3p and MGAT1 by dual-luciferase reporter assay. (c) RT-qPCR was used to quantify MGAT1 mRNA in U2OS and saos-2 cells treated with the miR-331-3p mimic or inhibitor, respectively. (d) WB showed MGAT1 expression in U2OS and saos-2 cells after transfected with the miR-331-3p mimic or inhibitor, respectively. *P < 0.05, **P < 0.01 and ***P < 0.001.