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. 2022 Jun 26;13(6):14159–14174. doi: 10.1080/21655979.2022.2083855

Figure 5.

Figure 5.

MGAT1 knockdown partially reduced the effect of miR-331-3p on U2OS cells. (a) Following MGAT1 siRNA transfection, RT-qPCR was utilized to quantify MGAT1 mRNA in U2OS cells. (b) U2OS cells proliferation following transfection with miR-331-3p inhibitor, MGAT1 siRNA, or miR-331-3p inhibitor and MGAT1 siRNA co-transfection, was assessed using the MTT assay. (c and d) Utilization of transwell for assessment of U2OS cells migration and invasion capacities 72 hours after treated with the miR-331-3p inhibitor, MGAT1 siRNA and miR-331-3p inhibitor, or MGAT1 siRNA and miR-331-3p inhibitor co-transfection. (e) Cell migration was observed 48 hours after scratching the cell layers. *P < 0.05, **P < 0.01 and ***P < 0.001.