TABLE 2.
Adhesion of MC and GC isolates expressing identical pilin variants to Hec-1B cellsa
| Variant | Adhesiveness of strain:
|
|
|---|---|---|
| FA1090 | 8013 | |
| Nonpiliated | 0.003 (0.003) | 0.0004 (0.0004) |
| SB::Km | 0.22 (0.02) | 0.087 (0.007) |
| SC::Km | 0.05 (0.02) | 0.02 (0.003) |
| 308::Km | 0.14 (0.03) | 0.076 (0.006) |
| SA::Km | 0.01 (0.007) | 0.008 (0.002) |
| RM11::Km | 0.015 (0.006) | 0.005 (0.001) |
a
Each pilin fusion was introduced into strains 8013 and FA1090. Adhesiveness was defined as the ratio of cell-associated CFU to CFU in the supernatant after 4 h of incubation. Values correspond to the mean of at least five assays; standard deviations are given in parentheses. In both 8013 and FA1090, high-adhesion pilin variants SB::Km and 308::Km were responsible for adhesiveness at least four to five times higher than that of low-adhesion variants SC::Km, SA::Km, and RM11::Km.