Figure 6.

Depletion of Nck1 and/or Nck2 triggers accrual of α actinin-4 and focal adhesion componentry in mouse glomeruli. (A) Immunoblotting (IB) and (B) densitometric quantification of glomerular lysates from 5-week-old control and podo-dKO mice. *P<0.05, **P<0.01, Mann–Whitney two-tailed t test. (C) Dual immunofluorescence microscopy of kidney sections stained for α actinin-4 and nephrin in control and podo-dKO mice. Scale bar, 100 μm. (D) IB and (E) densitometric quantification of glomerular lysates from 12-month-old WT, Nck1 KO, and Nck2 KO animals. *P<0.05, **P<0.01, Mann–Whitney two-tailed t test. (F) Immunofluorescence microscopy of kidney sections stained for α actinin-4 and nephrin in WT, Nck1 KO, and Nck2 KO mice.