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. 2022 Aug 1;11(8):e1407. doi: 10.1002/cti2.1407

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© 2022 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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The integrity of intestinal epithelial monolayers from Crohn's disease (CD) patients is compromised compared to those from non‐inflammatory bowel disease (non‐IBD) donors. (a) Scheme of the generation of intestinal monolayers (created in Biorender). (b) Representative H&E images of non‐IBD (left) and CD donor (right) monolayers (representative of 10 non‐IBD and 8 CD). (c) Non‐IBD and CD donor intestinal monolayers were cultured for 9 days in nondifferentiation media (NDM), then for 3 days in differentiation media (DFM). Transepithelial electrical resistance (TEER) readings were taken at indicated intervals. Vertical dotted line represents the change from NDM to DFM. Non‐IBD (n = 5), CD (n = 6). Statistical analyses were calculated using a mixed‐effects model and Sidak's multiple comparisons test (***P < 0.001, ****P < 0.0001).