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. 2022 Aug 1;17(8):e0267370. doi: 10.1371/journal.pone.0267370

Fig 3. Purification of membrane translocated mature FopA.

Fig 3

(A) The Ni-NTA elution profile and (B) SEC profile of FopA. (C) SDS-PAGE of FopA purification products visualized by Coomassie stain. Lane a, pooled elution fractions of FopA from Ni-NTA affinity chromatography; Lane b, pooled elution fractions of mature FopA (elution volume 10–14 mL) in SEC; lane c, pooled elution fractions of truncation products of FopA (elution volume 16–17 mL) in SEC. (D) Immunoblot using anti-FopA polyclonal antibody of the same samples as in C.