Fig 4. Assessing the oligomeric state and relative molecular weight (Mr) of FopA.
(A) Distribution of the various oligomeric states of FopA following first size exclusion chromatography (SEC). Elution peaks containing mature FopA are designated as Ve1 and Ve2. (B) Clear native-PAGE of the elution peaks of the first SEC. (C) Chromatogram of a second SEC that was loaded with peak Ve2 from the first SEC in A. (D) SEC column calibration using known protein standards (black squares) and calculated Mr of dimeric FopA (open circle) eluted during the second SEC.