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. 2022 Aug 1;17(8):e0267370. doi: 10.1371/journal.pone.0267370

Fig 5. Secondary structural analyses of purified FopA.

Fig 5

(A) Circular dichroism spectroscopy of membrane translocated FopA. The far-UV spectrum shows characteristic minimum and maximum ellipticities near 218 and 196 nm, respectively the characteristic values for β-strand-rich proteins. (B) Heat modifiability of FopA tested by SDS-PAGE stained with Coomassie blue. Unfolded FopA runs at the expected theoretical MW of mature FopA-His8 (40.2 kDa), while the folded FopA runs at an apparent MW of ~35 kDa.