TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or phenotype | Reference |
|---|---|---|
| Strains | ||
| H. seropedicae SMR54 | nifA | 27 |
| A. brasilense | ||
| FP2 | Nif+ | 23 |
| FP9 | ntrC | 23 |
| FP10 | nifA | 23 |
| E. coli ET8894 | lacZ ntrC | 10 |
| Plasmids (vector) | ||
| pDK7(pBR322) | CmrlacIq, overexpression from ptac | 17 |
| pRT22(pACYC177) | CmrK. pneumoniae nifH-lacZ | 27 |
| pEMS130(pTZ18) | CbrH. seropedicae nifA | This work |
| pEMS131(pTZ18) | CbrH. seropedicae ΔRnifA | This work |
| pEMS135(pLAFR3.18) | TcrH. seropedicae nifA | This work |
| pEMS136(pLAFR3.18) | TcrH. seropedicae ΔRnifA | This work |
| pNH11(pBR322) | Cbr ptac-nifA (K. pneumoniae) | 13 |
| pTZ18 | Cbr | 19 |
| pLAFR3.18 | Tcr IncP cosmid with pTZ18 cloning nest | 28 |