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. 2022 Jun 27;10:4300208. doi: 10.1109/JTEHM.2022.3186836

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FIGURE 1. — (A): Design principles of a nanobiosensor for protease detection. The OFF mode occurs when distance between fluorophore TCPP (tetrakis-carboxyphenyl-porphyrin), Fe/Fe₃O₄ nanoparticle, and FRET-acceptor cyanine (Cy) 5.5C is reduced, upon cleavage of the oligopeptide tether by a suitable protease present, this distance increases and leads to an increase in fluorescence intensity, which is called the ON mode. (B): TEM and HRTEM of dopamine-coated Fe/Fe₃O₄ core/shell nanoparticles. (C): Typical emission spectra occurring from a nanosensor for protease detection after 1h of incubation at 37 ⁰C ( nm). low: buffer; middle: nanosensor; high: nanosensor after incubation with the respective enzyme; with permission from reference [22], copyright Elsevier, Amsterdam 2021.

Inline graphic — (A): Design principles of a nanobiosensor for protease detection. The OFF mode occurs when distance between fluorophore TCPP (tetrakis-carboxyphenyl-porphyrin), Fe/Fe₃O₄ nanoparticle, and FRET-acceptor cyanine (Cy) 5.5C is reduced, upon cleavage of the oligopeptide tether by a suitable protease present, this distance increases and leads to an increase in fluorescence intensity, which is called the ON mode. (B): TEM and HRTEM of dopamine-coated Fe/Fe₃O₄ core/shell nanoparticles. (C): Typical emission spectra occurring from a nanosensor for protease detection after 1h of incubation at 37 ⁰C ( nm). low: buffer; middle: nanosensor; high: nanosensor after incubation with the respective enzyme; with permission from reference [22], copyright Elsevier, Amsterdam 2021.