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. Author manuscript; available in PMC: 2023 Jul 21.
Published in final edited form as: Cell. 2022 Jul 21;185(15):2739–2755. doi: 10.1016/j.cell.2022.06.031

Figure 1. Approaches to characterize cell types.

Figure 1.

(A) Molecular and anatomical approaches as primary ways of single-cell characterization include single-cell transcriptomics by sc/snRNA-seq, single-cell epigenomics exampled by snATAC-seq, spatially resolved transcriptomics exampled by MERFISH, full morphology reconstruction exampled by MouseLight (image adopted from Winnubst et al., 2019), EM connectomics (image adopted from Hulse et al., 2021), and barcoded connectomics exampled by BARseq (image adopted from Chen et al., 2019). (B) Cross-modality integrated approaches include Patch-seq (image adopted from Lee et al., 2021), retrograde tracing followed by molecular profiling, functional imaging followed by spatially resolved transcriptomics, using Patch-seq data as a Rosetta stone to assign molecular identities to neurons reconstructed from EM dataset (image adopted from Turner et al., 2022), and generation of enhancer based viral vectors (image adopted from Mich et al., 2021).