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. 2022 May 2;189(4):2467–2480. doi: 10.1093/plphys/kiac195

Figure 1.

Figure 1

BBX11 expression is induced by UV-B. A, RT-qPCR analysis of BBX11 expression in 5-day-old Col-0 seedlings grown in constant white light and exposed to supplemental UV-B 2 (2.2 W/m2) (+UV-B) or not (−UV-B) for the indicated time period. GAPDH was used as internal control. Error bars represent sem, n = 3 (B) Histochemical staining of 5-day-old pBBX11:GUS seedlings grown in constant white light and exposed to supplemental UV-B (+UV-B) or not (−UV-B) for the indicated durations, Scale bar- 500 μm. In (A and B) fluence of UV-B used was 2.2 W/m2 (C) RT-qPCR analysis of BBX11 expression in 5-day-old Col-0 seedlings grown in constant white light and exposed to supplemental UV-B of different doses UV-B 1 (0.91 W/m2), UV-B 2 (2.2 W/m2), UV-B 3 (3.9 W/m2), or not (−UV) for 6 h. GAPDH was used as internal control. Error bar represents sem, n = 3, Statistical groups indicated by letters were determined by one-way ANOVA, with Tukey’s test P ≤ 0.05. D, Histochemical staining of 5-day-old pBBX11:GUS seedlings grown in constant white light and exposed to supplemental UV-B 1, UV-B 2, UV-B 3, or not (−UV) for 10 h before staining. Scale bar: 500 μm.