Figure 4.

Auxin–cytokinin homeostasis control of S. hermonthica meristem activity. A–D′, immunolocalization showing auxin distribution in S. hermonthica root sections using anti-IAA antibody, N = 30; E–H′, cytokinin accumulation using anti-cytokinin antibody. Seedlings are 24 (A and E) and 48 h (B and F) after GR24 treatment-induced germination and 12 h (C, G, D, and H) of rice extract treatment. N = 20; A–D and E–H are fluorescence channel; A′–D′ and E′–H′ are overlay fluorescence and transmission light channels. I–K, IAA application delayed meristem differentiation in the S. hermonthica radicle, as monitored by EdU staining. I and J, Confocal images of control (I) and IAA-treated roots (J). MQ, Milli-Q water. K, Percentage of root containing cells undergoing cell division (EdU-stained cells) in S. hermonthica roots treated with different molar IAA concentrations. Columns indicate the means generated from three biological replicates; error bars represent se. A significant difference from the value of the non-treated roots versus IAA-treated roots was determined by t test (P < 0.05). The number of S. hermonthica seedlings used were as follows for the control (N = 44, 53, 40); 10⁻⁸ (N = 43, 45, 48); 10⁻⁷ (N = 93, 107, 95). N represents the number of seedlings used from three independent biological replicates. All scale bars: 50 µm.