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. 2022 May 4;189(4):2517–2534. doi: 10.1093/plphys/kiac197

Figure 3.

Figure 3

Al stress decreases ANAC017 protein accumulation. A, Fluorescence of ANAC017 fused GFP in root tip region (A), the elongation zone with central view (B) and the elongation zone with surface view (C) in ANAC017-GFP transgenic plants under control and Al treatment. Cell wall was stained with PI dye (red). Scale bars: 50 µm. Data shown are means ± sd of three biological replicates. Asterisks above the bars indicate significant differences at P < 0.05 by Student’s t test. D, Relative protein level of ANAC017-GFP under −Al or +Al conditions. Four-week-old seedlings of two ANAC017-GFP transgenic lines (ANAC017-GFP-1 and ANAC017-GFP-2) were treated without Al or with 50 μM Al for 24 h and roots were sampled for immunoblot analysis of GFP and Actin control.