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. 2022 May 4;189(4):2517–2534. doi: 10.1093/plphys/kiac197

Figure 4.

Figure 4

Cell wall composition and its Al binding capacity analysis in WT and anac017 mutants. A, Al content in WT and anac017 mutants root cell wall. Cell walls were extracted from roots of 4-week-old WT and anac017 mutants with Al for 24 h. Data are means ± sd (n = 4). Different letters above the bars indicate significant differences at P < 0.05 by one-way ANOVA analysis. B, Al adsorption kinetics of WT and anac017 mutants cell wall. Cell walls were extracted from 4 -week-old WT and anac017 mutants roots without Al. C, Al content in the root cell wall hemicellulose of WT and anac017 mutants. Cell walls were extracted from roots of 4-week-old WT and anac017 mutants with Al for 24 h. Data are means ± sd (n = 4). Different letters above the bars indicate significant differences at P < 0.05 by one-way ANOVA analysis. D, Total sugar content in the root cell wall hemicellulose of WT and anac017 mutants. Seedlings were treated with or without Al for 24 h. Data are means ± sd (n = 4). Different letters above the bars represent significant differences at P < 0.05 by one-way ANOVA analysis. E, The relative abundance of xyloglucan oligosaccharides by the MALDI-TOF MS analysis. Cell walls were extracted from Col-0 and anac017 mutants roots in the absence of Al and digested with XEG. The asterisk shows a significant difference between WT and anac017 mutants at P < 0.05 by Student’s t test.