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Transcriptional activation assay of ANAC017 protein in yeast. A, Schematic diagram of the constructs used for transcriptional activation assay in yeast. ANAC017 was truncated to generate four fragments including that encodes full-length cDNA. B, Transcriptional activation assay in yeast. Growth of yeast cells in medium SD/–Trp (left) and SD/–Trp–His–Ade (right) containing X-gal.
