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. 2022 May 4;189(4):2517–2534. doi: 10.1093/plphys/kiac197

Figure 8.

Figure 8

Regulation of XTH31 by ANAC017 TF. A, A pair of plasmids, pAbAi-pXTH31, pAbAi-pXTH17, pAbAi-pXTH15, and pGADT7-ANAC017 were introduced into Y1HGold and cultured on SD medium without Ura containing AbA (200 ng/mL; AbA200) or not (−AbA) at 30°C for 3 days. A pair of plasmids pAbAi-p53 and pGADT7-p53 were used as the positive control. B and C, The expression of XTH31 in WT, anac017 mutants, and ANAC017 overexpression lines in the WT background were detected with or without Al toxicity. D, A schematic of the XTH31-promoter-reporter construct, the effector plasmid, and the transfection control plasmid. Relative LUC activity of pXTH31-LUC reporter after co-expression of p35S:ANAC017 in Arabidopsis protoplasts. 35S-empty vector was used as the effector plasmid control. Error bars represent ± sd (n = 3). Asterisks above the bars indicate significant differences at P < 0.05 by Student’s t test. E and F, Diagram of XTH31 promoter showing five motifs. XF1-2 indicates genomic DNA fragments. The lgG was used as negative control. Error bars indicate ±sd (n = 3).