TABLE 2.

Regulation of aniC and aciK (hyaB) by pH, anaerobiosis, tyrosine, nitrate, and formate

Strain	Genotype	β-Galactosidase activity (Miller units)a
		Aerobic, pH 5.8, tyrosineb	Anaerobic
			pH 7.7, tyrosine		pH 5.8
					No tyrosine		Tyrosine
			No KNO3	KNO3c	No formate	Formated	No addition	Formate	KNO3
JF3306	aniC::MudJ	0	6	21	0	0	333	350	172
JF3307	hyaB::MudJ	0	0	17	0	0	318	331	13

^aCells were grown in Vogel and Bonner E minimal medium supplemented with 0.4% glucose (EG media) (35). Anaerobiosis was achieved by paraffin oil overlay. 

^bTyrosine was added at 100 μM. 

^cPotassium nitrate (KNO₃) was added at 0.5%. 

^dSodium formate was added at 0.1%.