TABLE 3.
Regulation of aniC-lacZ and aciK (hyaB)-lacZ by rpoS, crp, atrE, and tyrR
| Strain | Genotype | β-Galactosidase activity (Miller units)
|
|||
|---|---|---|---|---|---|
| pH 7.7a
|
pH 5.8
|
||||
| − Tyrosine | + Tyrosine | − Tyrosine | + Tyrosine | ||
| JF3306 | aniC::MudJ | 0 | 3 | 2 | 358 |
| JF3330 | aniC::MudJ atrE::Tn10 | 1 | 2 | 3 | 13 |
| JF3496 | aniC::MudJ tyrR::Tn10dTc | 2 | 2 | 1 | 0 |
| JF3338 | aniC::MudJ rpoSΩpRR10(Ap) | 0 | 5 | 1 | 738 |
| JF3307 | hyaB::MudJ | 0 | 0 | 0 | 318 |
| JF4053 | hyaB::MudJ tyrR::Tn10dTc | 0 | 0 | 0 | 0 |
| JF3331 | hyaB::MudJ atrE::Tn10 | 0 | 0 | 0 | 3 |
| JF3339 | hyaB::MudJ rpoSΩpRR10(Ap) | 0 | 0 | 0 | 476 |
| JF3586 | aniC::MudJ crp::Tn10 | 0 | 0 | 0 | 0 |
| JF3587 | hyaB::MudJ crp::Tn10 | 0 | 0 | 0 | 0 |
| JF3591 | aniC::MudJ cya::Tn10 | 0 | 0 | 0 | 6 |
| JF3582 | hyaB (aciK)::MudJ cya::Tn10 | 0 | 0 | 0 | 0 |
| JF3602 | aniC::MudJ atrE::Tn10/pJC100 (tyrR) | 0 | 0 | 0 | 13 |
| JF3603 | hyaB (aciK)::MudJ atrE::Tn10 | 0 | 0 | 0 | 0 |
| JF3618 | aniC::MudJ tyrR::Tn10dTc/pJC100 | 0 | 6 | 95 | 697 |
| JF3634 | aniC::MudJ fnr::Tn10 | 0 | 0 | 0 | 422 |
| JF3635 | hyaB (aciK)::MudJ fnr::Tn10 | 0 | 0 | 0 | 248 |
a
Cells were grown anaerobically in EG medium at pH 7.7 or pH 5.8 with or without tyrosine (100 μM).