Figure 6.

The synthesis of chloroplast-encoded thylakoid proteins in WT and nadk2-2 plants. A, Pulse labeling of thylakoid proteins of nadk2-2 and WT plants. After pulse labeling in the presence of cycloheximide for 20 min, thylakoid membranes were isolated from Arabidopsis leaves. The thylakoid proteins were separated by SDS–urea–PAGE and visualized autoradiographically. As a loading control, the same samples were separated on an additional gel and stained with CBB. The CBB-stained gel is shown on the right. B, Pulse-chase labeling of thylakoid membrane proteins from WT and nadk2-2 plants. After pulse labeling with [³⁵S]-Met for 20 min as in (A) but without cycloheximide, leaves were incubated with an excess of cold Met for an additional 1, 2, and 4 h. Labeled proteins were then visualized by subsequent autoradiography.