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. 2022 May 11;189(4):2382–2395. doi: 10.1093/plphys/kiac205

Figure 4.

Figure 4

NopT-trigged effects in Arabidopsis and N. benthamiana plants depend on PBS1 proteins and AtRPS5. A, Effects of NopT expressed in Arabidopsis leaves (Col-0 wild-type; pbs1-2 and rps5-2 mutants) 4 days postinfiltration with A. tumefaciens carrying pCAMBIA2301 containing full-length nopT of NGR234 or the EV. NopT-triggered leaf chlorosis was only observed in wild-type plants. Agroinfiltration holes are marked by circles. B, Reconstitution of the AtPBS1/AtRPS5 pathway in N. benthamiana. Agrobacterium-mediated transformation was used to co-express constructs encoding NopT (lacking residues 1–48) or variants, AtPBS1, and AtRPS5. The EV pTA7002 was used as a control. Protein expression was induced by 50 µM DEX (42 h postagroinfiltration) and leaves were photographed 48 h later. The pictures in the lower panel were taken with a UV camera to illustrate formation of necrotic tissue. C, Replacement of AtPBS1 by GmPBS1-1 showed similar effects when NopT proteins and AtRPS5 were co-expressed.