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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Nat Methods. 2022 May 9;19(6):671–674. doi: 10.1038/s41592-022-01478-3

Table 1.

Evaluated metagenome datasets

Sample Accession # bases (Gb) N50 read length (kb) Median read QV Sample description
ATCC SRR11606871 59.2 12.0 36 Mock community ATCC MSA-100317
zymo SRR13128014 18.0 10.6 40 Mock community ZymoBIOMICS D6331
sheepA SRR10963010 51.9 14.3 25 Sheep gut microbiome5
sheepB SRR14289618 206.4 11.8 N/A Sheep gut microbiome14
chicken SRR15214153 33.6 17.6 30 Chicken gut microbiome
sludge ERR7015089 15.3 15.4 32 Anaerobic digester sludge
humanO1 SRR15275213 18.5 11.4 40 Human gut from a pool of four omnivore samples
humanO2 SRR15275212 15.5 10.3 41 Human gut from a pool of four omnivore samples
humanV1 SRR15275211 18.8 11.0 39 Human gut from a pool of four vegan samples
humanV2 SRR15275210 15.2 9.6 40 Human gut from a pool of four vegan samples

The N50 read length is the length of the shortest read at 50% of the total number of read bases. The quality value (QV) of a read is −10log10e, where e is the expected sequencing error rate of the read, assuming accurate base quality. No base quality is available for the sheepB dataset.