FIG. 2.

Expression of the Cap1p and Cap1p-TR proteins in C. albicans. (A) Schematic representation of the full-length (Cap1p) and the truncated (Cap1p-TR) proteins. Positions of the bZip domain (grey) and the CRD (stippled) are indicated. The region used to generate a GST-Cap1p fusion protein (Cap1-350) is indicated. This fusion protein was used to raise an anti-Cap1p-350 polyclonal antibody. (B) Western blot analysis of Cap1p and Cap1p-TR expression. Total proteins were extracted from CAI4, CJD21, CJD21/PMK, CJD21/PMK-CAP1, and CJD21/PMK-CAP1TR cells. Protein samples (50 μg) were separated by electrophoreses on an SDS–12% polyacrylamide gel, transferred to a nitrocellulose membrane, and analyzed with the anti-Cap1p-350 polyclonal antibody. Arrows on the right indicate positions of the full-length (Cap1p) and truncated (Cap1p-TR) proteins. The position of a nonspecific cross-reacting protein is also indicated (★). Positions of molecular size standards are shown on the left.