FIG. 4.

Northern blot analysis of CAP1 transcriptional targets in C. albicans. Total RNA was extracted from strains CAI4, CJD21, CJD21/PMK, CJD21/PMK-CAP1, and CJD21/PMK-CAP1TR. RNA samples (20 μg) were separated by electrophoresis on a 1% agarose gel, transferred onto nylon membranes, and probed with a CAP1, MDR1, CaYCF1, CaGLR1, or CaTRR1 probe. Positions of rRNAs are indicated on the left; the position of the major CAP1 transcript is indicated by an arrow (top panel). The membranes were exposed for either 24 h (CAP1), 3 days (CaYCF1, CaGLR1, and CaTRR1), or 12 days (MDR1) at −80°C with two intensifying screens.