FIG. 5.

Northern blot analysis of CAP1 and MDR1 in FR2 cap1 disruptants. Total RNA was extracted from strains SGY243, FR2, FJD11 (CAP1/cap1Δ::hisG), and FJD21 (cap1Δ::hisG/cap1Δ::hisG). RNA samples (10 μg) were separated by electrophoresis in duplicate on a 1% agarose gel and transferred onto nylon membranes. The membranes were probed with a CAP1 (A) or MDR1 (B) probe. Both blots were subsequently hybridized with an ACT1 probe as a control for RNA loading and transfer. Membranes were exposed for 10 days (MDR1 and CAP1) or 24 h (ACT1) at −80°C with two intensifying screens. Positions of rRNAs are indicated on the left.