Fig. 4.

Downregulated miR-21-5p in M2 macrophage-derived EVs suppresses PaCa stem cell differentiation and activity. a RT-qPCR detection of miR-21-5p expression after P-CSCs and A-CSCs co-culturing with EVs, b RT-qPCR detection Nanog and Oct4 expression, c tumor sphere formation assay of cell sphere-forming ability, d colony formation assay of cell colony-forming ability, e flow cytometry detection of cell apoptosis, and f Transwell assay detection of cell migration and invasion. *P < 0.05 compared with the NC-EV group; #P < 0.05 compared with the miR-21-5p-EV group. N = 3; The data were expressed as mean ± standard deviation. The disparities among multiple groups were evaluated by one-way ANOVA, followed by Tukey’s multiple comparisons test