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. 2021 Dec 20;43(8):2139–2146. doi: 10.1038/s41401-021-00793-3

Fig. 1. Effect of 35 compounds on cell viability and hPXR activation.

Fig. 1

a The skeletons of compounds in Euphorbiaceae diterpenoid library. b The cytotoxicity of compounds 1–35 (10 μM) on HEK293T cells. c Effect of compounds 1–35 on hPXR activation. Dual-luciferase reporter assays were performed in HEK293T cells transiently transfected with expression plasmid (pSG5-hPXR), reporter plasmid (pGL3-CYP3A4-XREM-Luc), and the control plasmid (pGL4.74-TK). Cells were treated with the positive agonist RIF (10 μM) or compounds 1–35 (10 μM) for 24 h. Data are presented as means ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001, versus the vehicle.