Skip to main content
. 2022 Aug 1;12:13202. doi: 10.1038/s41598-022-17269-x

Figure 1.

Figure 1

IMhu cytokine response to stimulation of major PRRs. Concentrations of TNF-α, IFN-α/β, IL-1β, IL-6, and IL-18 in the supernatant of IMhu cells 24 h following stimulation with 5 μg/ml dsDNA and 1.5 μl/ml L3000; 1 µM CpG ODN and 5 µl/ml LTX; 10 μg/ml Poly I:C; or 5 μg/ml LPS and 5 mM ATP. Cytokine concentrations were measured using HEK-Blue IFN α/β, IL-6, TFNα, IL-1β, and IL-18 reporter cells. Error bars represent means + SD of n = 3 per group. Normally distributed data was assessed using One-way ANOVA with post hoc Tukey’s tests. Non-normally distributed data was assessed using non-parametric Mann–Whitney tests or Kruskal–Wallis tests with post hoc assessment using Dunn’s tests with control for joint ranks and Bonferroni adjustment. *p < 0.05, **p < 0.01. Asterisks indicate significant differences in comparison to unstimulated cells.