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. 2021 Dec 22;43(8):2081–2093. doi: 10.1038/s41401-021-00833-y

Fig. 6. 11R-VIVIT attenuates tubular epithelial cell apoptosis in an animal model of AKI-to-CKD progression.

Fig. 6

a TUNEL staining of frozen renal tissue sections from the sham-operated, IRI and IRI + 11R-VIVIT treatment groups on the 2nd, 14th and 28th day. Scale bars = 50 μm. b Quantification of TUNEL‑positive tubular epithelial cells in the sham-operated, IRI and IRI + 11R-VIVIT treatment groups on the 2nd, 14th and 28th day. c Western blot analysis of the expression of caspase-3 and C‑caspase-3 in each group on the 2nd, 14th and 28th day. d–e The quantitative results of C-caspase-3 and caspase-3 were normalized to β-actin. f Western blot analysis of the expression of Bax in each group on the 2nd, 14th and 28th day. g The quantitative results of Bax were normalized to β-actin. *P < 0.05 vs. IRI-2d, #P < 0.05 vs. IRI-14d, &P < 0.05 vs. IRI-28d. AKI acute kidney injury, CKD chronic kidney disease, RTECs renal tubular epithelial cells, IRI ischemia-reperfusion injury, C‑caspase‑3 cleaved-caspase‑3.