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. 2022 Aug 1;13(8):667. doi: 10.1038/s41419-022-05043-w

Fig. 2. Hepcidin regulates the levels of FPN1 of BMVECs.

Fig. 2

A is western blot analysis of FPN1 and ferritin levels in BMVECs 24 h following ICV injection of hepcidin into 3-month-old WT mice. B is the relative expression levels of FPN1 and ferritin in control and hepcidin-treated mice calculated after they were normalized to β-actin levels and expressed as the mean ± SEM. n = 3, **p < 0.01. C, D are the total iron in the cerebral cortex (C), hippocampus (D) of Hamp KO or control WT mice of the indicated ages determined by ICP-MS. Values are presented as the mean ± SEM, n = 3, *p < 0.05 and **p < 0.01. E is the FPN1 protein levels in BMVECs of WT mice of the indicated ages. The relative expression levels were expressed as the mean ± SEM, n = 3, *p < 0.05 vs. 1-week-old mice. F is the FPN1 protein levels in BMVECs of Hamp KO mice of the indicated ages. The relative expression levels were expressed as the mean ± SEM, n = 3, *p < 0.05 and **p < 0.01 vs. 1-week-old mice. G is the protein levels of FPN1 in BMVECs measured by western blot analysis following ICV injection of hepcidin in 24-week-old Hamp KO and WT mice. The relative expression levels were normalized and expressed as the mean ± SEM, n = 3, **p < 0.01 and ***p < 0.001. H is total iron level in BMVECs determined by ICP-MS in 24-week-old Hamp KO and WT mice. The values are presented as the mean ± SEM, n = 3, *p < 0.05 vs. WT group.