TABLE 1.
Bacterial strain and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strain | ||
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 lac/[F′::Tn10 proAB+ lacIqlacZM15 traD36] | 2 |
| Plasmids | ||
| pBluescriptII SK+ | Apr ColE1 replicon, cloning vector | Stratagene |
| pSTV29 | Cmr pACYC replicon, cloning vector | 4, 25 |
| pTUE1122 | Aprtac promoter, 6xHis, expression vector | 16 |
| pMR26 | Broad-spectrum mercury resistance plasmid from Pseudomonas strain K-62 | 8, 10 |
| pMRA17 | 6.6-kb SacI fragment of pMR26 containing merRTPAGB cloned into pBluescriptII SK+ | 8, 10 |
| pMR96 | 4.7-kb BglII-EcoRV fragment of pMRA17 containing merRTPAGB cloned into pBluescriptII SK+ | This study |
| pMC89 | merB-deleted plasmid, containing merRTPAG, constructed from pMR96 | This study |
| pMU29 | merG-deleted plasmid containing merRTPAB cloned into pBluescriptII SK+ | 10 |
| pMRD141 | merAGB-deleted plasmid containing merRTP, constructed from pMRA17 | 9, 10, 31 |
| pMEV89 | 1.2-kb HindIII-BamHI fragment of pMC89 containing merG cloned into pSTV29 | This study |
| pMUE74 | 697-bp PCR products of merG cloned into pTUE1122 | This study |