Fig. 4. Ddx3xb phase separation facilitates zebrafish MZT.

a Cumulative distribution and boxplots (inside) of the log₂ fold changes of RNA levels of ddx3xb mutant versus wild-type embryos in maternal decay, maternal stable, and zygotic groups at 6 hpf. P values were calculated by the Kruskal Wallis test. b Cumulative distribution and boxplots (inside) of the log₂ fold changes of RNA levels of maternal decay group in ddx3xb wild-type and mutant embryos during the period of 2 hpf to 6 hpf. P values were calculated by the Kruskal Wallis test. c Cumulative distribution of the log₂ fold changes of RNA levels of zygotic group in ddx3xb wild-type and mutant embryos during the period of 2 hpf to 6 hpf. P values were calculated by the Kruskal Wallis test. d Gene set enrichment analysis of downregulated genes in the zygotic group compared between ddx3xb mutant and wild-type embryos at 6 hpf. e Gene set enrichment analysis of upregulated genes in the maternal decay group compared between ddx3xb mutant and wild-type embryos at 6 hpf. f Cumulative distribution and boxplots (inside) of the log₂ fold changes of RNA levels between ddx3xb_FL and ddx3xb_△N mRNAs-injected ddx3xb mutant embryos in maternal decay, maternal stable, and zygotic groups at 6 hpf. P values were calculated by the Kruskal Wallis test. g Cumulative distribution and boxplots (inside) of the log₂ fold changes of RNA levels of maternal decay group in ddx3xb_FL and ddx3xb_△N mRNAs-injected ddx3xb mutant embryos during the period of 2 hpf to 6 hpf. P values were calculated by the Kruskal Wallis test. h Cumulative distribution and boxplots (inside) of the log₂ fold changes of RNA levels of zygotic group in ddx3xb_FL and ddx3xb_△N mRNAs-injected ddx3xb mutant embryos during the period of 2 hpf to 6 hpf. P values were calculated by the Kruskal Wallis test.