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. 2022 Jul 19;9:964177. doi: 10.3389/fvets.2022.964177

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Copyright © 2022 Urbani, Tirolo, Balboni, Troia, Dondi and Battilani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree based on the complete VP2 gene nucleotide sequences of canine parvovirus type 2 (CPV-2) obtained in this study and reference strains retrieved from GenBank database. Phylogeny was constructed by MEGA 11 version 11.0.11 using Neighbor-Joining method and the Tamura 3-parameters (T92 model with gamma distribution. Statistical support was provided by bootstrapping with 1,000 replicates. Bootstrap values >80% are indicated on the respective branches. Highlighted in black: Sequences generated in this study (only the viral sequence identified in Dog 1 is reported because the nucleotide sequences obtained from the two dogs were identical). The CPV-2 antigenic variants and the amino acid residues in positions 5, 267, 324 and 370 of deduced VP2 protein are reported for each cluster.