Table 1.
Primers used for molecular detection of viral, bacterial and parasitic DNA and viral RNA.
| Pathogen | Primer name | Primer sequence | DNA/RNA fragment amplified | References |
|---|---|---|---|---|
| CPV-2 | VP2_For | 5'-ACCAACTAAAAGAAGTAAACCA-3' | VP2 gene (1,885 bp) | (9) |
| VP2_Rev | 5'-GTAATAAACATAAAAACATAGTAAGTA-3' | |||
| Anaplasma spp. | groEL_For | 5'-ACTGATGGTATGCARTTTGAYCG-3' | groEL gene (about 600 bp) | (47) |
| Ehrlichia spp. | groEL_Rev | 5'-TCTTTRCGTTCYTTMACYTCAACTTC-3' | ||
| CAdV-1 CAdV-2 | CAdV-qPCR_For3 | 5'-CTGASACTGCWATRMCTATATAYATTTCCA-3' | E3 gene (166 bp) | (14) |
| CAdV-qPCR_Rev2 | 5'-GACATAGARACRCAGGACCCAGA-3' | |||
| CanineCV | CaCV-qPCR_For | 5'-CTGAAAGATAAAGGCCTCTCGCT-3' | IR between the ends of the two major ORFs (132 bp) | (16) |
| CaCV-qPCR_Rev | 5'-AGGGGGGTGAACAGGTAAACG-3' | |||
| Leptospira spp. | LipL32_For | 5'-AAGCATTACCGCTTGTGGTG-3' | LipL32 gene (242 bp) | (13) |
| LipL32_Rev | 5'-GAACTCCCATTTCAGCGATT-3' | |||
| Babesia spp. | B-lsu_For | 5'-ACCTGTCAARTTCCTTCACTAAMTT-3' | B-lsu gene (about 150 bp) | (15) |
| B-lsu_Rev2 | 5'-TCTTAACCCAACTCACGTACCA-3' | |||
| CDV | qCDVFor4 | 5'-GTCGGTAATCGAGGATTCGAGAG-3' | P gene (114 bp) | (17) |
| qCDVRev3 | 5'-GCCGAAAGAATATCCCCAGTTAG-3' |
CAdV-1, canine adenovirus type 1; CAdV-2, canine adenovirus type 2; CanineCV, canine circovirus; CDV, canine distemper virus; CPV-2, Canine parvovirus type 2; IR, intergenic region; ORFs, open reading frame.