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. 2022 Jul 19;10:959521. doi: 10.3389/fcell.2022.959521

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Copyright © 2022 Qin, Melamed, Yang, Saxena, Sheetz and Wolfenson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of DAPK1 contributes to cell growth on soft matrices. (A) Bright field images of MEFs with DAPK1 inhibitor or stable DAPK1 shRNA MEFs on stiff or soft surfaces. Scale bar: 25 μm. (B) MEF cells with DAPK1 inhibitor or stable DAPK1 shRNA MEFs were incubated in culture for 1 day on soft or stiff surfaces. Apoptotic cells were identified for appearance of apoptotic morphology including membrane blebbing and cell rounding. Live cells retained their normal spread morphology. Graphs represent means ± SEM of at least two independent experiments. ***p < 0.001. (C) MEF cells were plated on 0.2 kPa gels and the levels of pS308 DAPK1 (pDAPK) and total DAPK1 (tDAPK) were monitored by double immunostaining of the cells over time. Graphs represent means ± SD. Experiment was repeated twice. ***p < 0.001.