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. 2022 Jul 19;10:959521. doi: 10.3389/fcell.2022.959521

FIGURE 7.

FIGURE 7

DAPK1 apoptotic activity is controlled by Src phosphorylation and PTPN12. (A) Left: Micrographs showing the distribution of DAPK1 in MEFs transfected with mCherry-paxillin and EGFP-DAPK1-DYD (phosphomimetic mutant), EGFP-DAPK1-DYF (non-phosphorylated mutant) or EGFP-DAPK1-DYF-K42A (non-phosphorylated inactive mutant) respectively after 30 min spreading on fibronectin-coated glass dishes. Scale bar: 10 μm. Right: Percentage of FA targeting positive cells were calculated from 200 transfected cells per group. ***p < 0.001. (B) MEFs were transfected with EGFP-DAPK1 WT, EGFP-DAPK1-DYD, EGFP-DAPK1-DYF or EGFP-DAPK1-DYF-K42A respectively for 1 day. The percentage of transfected cells that were still alive was quantified. The mean ± SEM of at least two independent experiments is described. For each experiment, 100–150 cells were analyzed for each transfection point. *p < 0.05, ***p < 0.001. (C) MEFs were transfected with EGFP-DAPK1 WT either co-transfected with mCherry-PTPN12 or treated with PTPN12 inhibitor. The percentage of co-transfected cells that were still alive was quantified. The mean ± SEM of at least two independent experiments is described. For each experiment, 100–150 cells were analyzed for each transfection point. **p < 0.01, ***p < 0.001.