Table 1.

Formulation and chemical composition of experimental diets (% dry matter).

Ingredients	PH (%)
	0	25	50	75	100
Pre-mixed protein ingredients a	70.73	53.05	35.36	17.68	0.00
Protein hydrolysates b	0.00	18.25	36.5	54.75	73.00
Fish protein powder c	4.00	4.00	4.00	4.00	4.00
Wheat gluten meal d	5.00	5.00	5.00	5.00	5.00
Sodium caseinate d	3.00	3.00	3.00	3.00	3.00
Yeast hydrolysate d	0.50	0.50	0.50	0.50	0.50
Starch d	3.00	3.00	3.00	3.00	3.00
Fish oil d	1.20	1.20	1.20	1.20	1.20
Soy Lecithin	5.00	5.00	5.00	5.00	5.00
Soybean oil d	2.00	2.00	2.00	2.00	2.00
$\text{Ca}{\left({\text{H}}_2{\text{PO}}_3\right)}_2^{\text{d}}$	1.00	1.00	1.00	1.00	1.00
Vitamin mixture e	1.30	1.30	1.30	1.30	1.30
Mineral mixture f	1.00	1.00	1.00	1.00	1.00
Microcrystalline cellulos e	2.27	1.70	1.14	0.57	0
Proximate analysis (Mean values, % dry weight)
Crude protein	59.22	59.31	59.42	59.36	59.30
Crude lipid	13.12	13.04	13.22	13.30	13.18
Ash	16.11	16.34	16.34	16.42	16.73
DH (% crude protein) g	11.16	18.04	23.73	28.52	37.57

^aThe composition of Premixed protein is shown in Supplementary Table1 ;

^bProtein hydrolysate is made by Premixed protein;

^cSupplied by Weifang Xipu Biotechnology Co., Ltd;

^dSupplied by Zhejiang Xinxin Tian’en Aquatic Feed Corporation (Jiaxing, China): wheat gluten meal, crude protein, 80.05%; crude lipid, 1.13%;

^eVitamin Premix (mg/kg diet): vitamin A, 16000 IU; vitamin B1, 17.80; vitamin B2, 48; vitamin B6, 29.52; vitamin B12, 0.24; vitamin C, 800; vitamin D3, 8000 IU; vitamin E, 160; vitamin K3, 14.72; choline chloride, 1500; niacinamide, 79.20; inositol, 320; calcium-pantothenate, 73.60; biotin, 0.64; folic acid, 6.40;

^fMineral Premix (mg/kg diet): I (Ca (IO₃)₂), 1.63; Cu (CuSO₄), 2.00; Fe (FeSO₄), 21.10; Co (CoCl₂), 0.24; Zn (ZnSO₄), 34.4; Se (Na₂SeO₃), 0.18; Mn (MnSO₄), 6.20.

^gDH, Degree of hydrolysis.